沉默CDK7通过下调YAP表达诱导子宫内膜癌细胞凋亡

doi:10.3969/j.issn.2095-1264.2023.06.11

首页 > 过刊浏览>2023年第6期 >729-734. DOI:10.3969/j.issn.2095-1264.2023.06.11

沉默CDK7通过下调YAP表达诱导子宫内膜癌细胞凋亡
DOI:
                        10.3969/j.issn.2095-1264.2023.06.11
                    
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作者单位:四川大学华西医院广安医院 妇产科，四川 广安，638500
作者简介:杨姣，女，硕士研究生，主治医师，研究方向：妇科肿瘤学。
通讯作者:陈会霞，女，硕士研究生，主治医师，研究方向：妇科肿瘤学。
中图分类号:R737.33
基金项目:

Silencing CDK7 promotes the apoptosis of endometrial cancer cells by downregulating the expression of YAP

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Affiliation:

Department of Obstetrics and Gynecology, West China-Guang'an Hospital, Sichuan University, Guang'an, 638500, Sichuan, China

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摘要:

目的研究沉默细胞周期蛋白依赖性激酶7（CDK7）诱导Yes相关蛋白（YAP）表达下调对子宫内膜癌HEC-1-A细胞凋亡的影响。方法利用siRNA干扰技术沉默HEC-1-A细胞中CDK7的表达，采用qPCR和Western blotting检测siRNA干扰后CDK7的表达水平，CCK-8实验检测沉默CDK7对HEC-1-A细胞活力的影响，流式细胞术检测沉默CDK7对HEC-1-A细胞凋亡的影响，Western blotting检测沉默CDK7对YAP和磷酸化YAP蛋白及其下游蛋白Cyr16、CTGF表达的影响，凋亡实验检测共转染si-CDK7和pcDNA3.1-YAP对细胞凋亡的影响。结果转染si-CDK7后，HEC-1-A细胞中CDK7 mRNA和蛋白表达水平均显著下降（P<0.01），细胞活性显著降低（P<0.01），细胞凋亡率显著升高（P<0.01），YAP和磷酸化YAP蛋白及其下游蛋白Cyr16、CTGF表达水平明显下降（P<0.01）；共转染pcDNA3.1-YAP可逆转沉默CDK7导致的细胞凋亡（P<0.01）。结论沉默CDK7可诱导YAP蛋白表达下调，促进子宫内膜癌HEC-1-A细胞凋亡。

Abstract:

Objective To investigate the effect of cyclin-dependent kinase 7 (CDK7) on the apoptosis of endometrial cancer HEC-1-A cell by regulating of Yes-associated protein (YAP) expression.Methods The CDK7 expression was silenced in HEC-1-A cells with the siRNA interference technique. Then, the mRNA and protein expression levels of CDK7 were detected by qPCR and Western blotting respectively. The viability and apoptosis of HEC-1-A cells were detected by CCK-8 and flow cytometry after silencing CDK7. Expression levels of YAP, p-YAP protein and its downstream proteins Cyr16 and CTGF were detected by Western blotting after silencing CDK7. The effect of co-transfection with si-CDK7 and pcDNA3.1-YAP on the apoptosis of HEC-1-A cells was detected by apoptosis assay.Results After transfection with si-CDK7, the mRNA and protein expression levels of CDK7 in HEC-1-A cells were decreased significantly (P<0.01), the activity of HEC-1-A cells was significantly decreased (P<0.01), the apoptosis rate was significantly increased (P<0.01), and the expression levels of YAP, pYAP protein and its downstream proteins Cyr16 and CTGF were significantly decreased (P<0.01). Co-transfection of pcDNA3.1-YAP reversed the apoptosis caused by silenced CDK7 (P<0.01).Conclusion Silencing CDK7 promoted the apoptosis of endometrial cancer HEC-1-A cells by downregulating the expression of YAP.