生物信息学分析OLFML2B基因在胃癌中的功能及临床预后价值<sup>★</sup>

doi:10.3969/j.issn.2095-1264.2023.04.11

首页 > 过刊浏览>2023年第4期 >466-474. DOI:10.3969/j.issn.2095-1264.2023.04.11

生物信息学分析OLFML2B基因在胃癌中的功能及临床预后价值★
DOI:
                        10.3969/j.issn.2095-1264.2023.04.11
                    
作者:
                        
                        
                    
作者单位:1.南华大学附属第二医院，消化内科，湖南 衡阳，421001;2.南华大学附属第二医院，临床医学研究中心，湖南 衡阳，421001
作者简介:欧阳佳凤，女，硕士研究生，研究方向：消化系统肿瘤防治研究。
通讯作者:李国庆，男，博士，教授，研究方向：消化系统肿瘤防治研究。
中图分类号:R735.2
基金项目:★湖南省卫生计生委2017年度第二批科研计划项目（A2017014）；2020年湖南省研究生科研创新项目（CX20200977）。

Functions and prognostic value of OLFML2B in gastric cancer: analysis based on data mining^★

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Affiliation:

1.Department of Gastroenterology, the Second Affiliated Hospital of the University of South China, Hengyang, 421001, Hunan, China;2.Clinical Research Center, the Second Affiliated Hospital of the University of South China, Hengyang, 421001, Hunan, China

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摘要:

目的采用生物信息学方法分析OLFML2B基因在胃癌中的表达、功能及预后价值。方法从Oncomine数据库挖掘胃癌中OLFML2B基因的表达信息，利用Kaplan-Meier plotter数据库分析OLFML2B在胃癌中的预后价值，并使用UALCAN数据验证胃癌中OLFML2B的表达水平及预后价值，最终通过LinkedOmics数据库探索胃癌中与OLFML2B相关的基因及可能的生物学过程。结果 Oncomine 数据库共纳入了319项OLFML2B在不同肿瘤中的研究结果，差异有统计学意义的共39项（均表现为OLFML2B在肿瘤中高表达），其中6项分析显示OLFML2B在胃癌中的表达较正常胃组织明显升高，包括324例样本，差异有统计学意义（Fold Change>2， P<0.000 1）。OLFML2B表达量与胃癌总体生存率呈负相关，即OLFML2B表达水平越高则患者总体生存率越低（P<0.05）。亚组分析发现，弥漫型胃癌与胃肠型胃癌患者中，OLFML2B高表达组总体生存率较低表达组明显缩短（P<0.05）。UALCAN数据库验证表明，OLFML2B在胃癌组织中高表达且与胃癌预后相关。此外，LinkedOmics数据库提示，OLFML2B可能作为癌基因，通过影响血管生成、细胞黏附和细胞周期等途径参与胃癌的发生发展。结论与正常胃组织相比，OLFML2B在胃癌组织中的表达水平明显升高，其高表达与胃癌患者不良预后相关，可能成为胃癌预后的生物标志物，并以癌基因的角色参与胃癌的发生发展。

Abstract:

Objective To explore the expression, function and clinical prognostic value of olfactomedin-like 2B (OLFML2B) in gastric cancer by bioinformatics methods.Methods The OLFML2B expression data were collected from Oncomine database. Correlation between the OLFML2B expression and the prognosis of gastric cancer were analyzed through Kaplan-Meier plotter database. Then the UALCAN online database was used to verify the expression and prognostic role of OLFML2B in gastric cancer. Finally, the genes associated with OLFML2B in gastric cancer and their possible biological processes were explored through LinkedOmics database.Results Of the 319 studies from Oncomine database analyzing the OLFML2B expression in different types of tumors, 39 studies showed statistical differences (OLFML2B expression was up-regulated in cancers in the 39 studies). Among them, 6 studies, which included 324 samples, revealed that OLFML2B was highly expressed in gastric cancer when compared with normal gastric tissue (fold change>2, P<0.000 1). Furthermore, the expression of OLFML2B was negatively correlated with the overall survival (OS) of gastric cancer patients, indicating that the high expression of OLFML2B was associated with the poor OS (P<0.05). Besides, subgroup analysis revealed that, in regard to diffuse or gastrointestinal gastric cancer patients, the survival time of OLFML2B-upregulated patients was shorter than that of OLFML2B-downregulated patients (P<0.05). The same upregulated status and prognostic role of OLFML2B in gastric cancer were confirmed in UALCAN database. Finally, LinkedOmics database suggested that OLFML2B may be involved in the development of gastric cancer via serving as an oncogene and influencing angiogenesis, cell adhesion, and cell cycle.Conclusions The expression of OLFML2B was significantly upregulated in gastric cancer tissue, and its high expression was associated with the poor prognosis of gastric cancer patients. OLFML2B has the potential to serve as a biomarker for prognostic prediction of gastric cancer. It may work as an oncogene to influence the occurrence and development of gastric cancer.