circ-E2F3促进宫颈癌细胞增殖的机制研究

doi:10.3969/j.issn.2095-1264.2023.04.07

首页 > 过刊浏览>2023年第4期 >443-448. DOI:10.3969/j.issn.2095-1264.2023.04.07

circ-E2F3促进宫颈癌细胞增殖的机制研究
DOI:
                        10.3969/j.issn.2095-1264.2023.04.07
                    
作者:
                        
                        
                    
作者单位:1.江阴市妇幼保健院，江苏 无锡，214400;2.南京市第二医院，江苏 南京，210003
作者简介:缪华珍，女，副主任药师，研究方向：医院药学。
通讯作者:
中图分类号:R737.33
基金项目:

Mechanism of circ-E2F3 promoting the proliferation of cervical cancer cells

Author:

Affiliation:

1.Jiangyin City Maternal and Child Health Hospital, Wuxi, 214400, Jiangsu, China;2.Nanjing Second Hospital, Nanjing, 210003, Jiangsu, China

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摘要:

目的研究circ-E2F3通过抑制miR-296-5p影响宫颈癌进展的作用机制。方法采用qRT-PCR检测宫颈癌组织和癌旁组织中circ-E2F3的表达量，筛选宫颈癌细胞系，通过qRT-PCR检测宫颈癌细胞系和正常宫颈黏膜上皮细胞中circ-E2F3的表达量。采用CCK-8检测细胞活力，通过检索数据库筛查circ-E2F3可能的分子机制，并通过双荧光素酶分析、qRT-PCR等方法证实其调控机制。结果 circ-E2F3在宫颈癌组织和宫颈癌细胞系CaSki中表达上调。体外实验证实circ-E2F3能促进宫颈癌细胞增殖；体内实验证实circ-E2F3通过抑制miR-296-5p促进肿瘤细胞生长。结论 circ-E2F3通过抑制miR-296-5p在宫颈癌的进展中发挥重要作用，高表达的circ-E2F3可能是判断宫颈癌进展的重要指标。

Abstract:

Objective To study the effect of circ-E2F3 on the progression of cervical cancer by inhibiting miR-296-5p.Methods The expression levels of circ-E2F3 in cervical cancer tissues and adjacent tissues were detected by qRT-PCR, and cervical cancer cell lines were then screened. The expression levels of circ-E2F3 expression in cervical cancer cell lines and normal cervical mucosal epithelial cells were detected by qRT-PCR. Cell viability was detected by CCK-8. The possible molecular mechanism of circ-E2F3 was screened by searching the database, and its regulatory mechanism was confirmed by dual luciferase analysis and qRT-PCR.Results The expression of circ-E2F3 was up-regulated in cervical cancer tissues and cervical cancer cell line CaSki. In vitro experiment found that circ-E2F3 can promote the proliferation of cervical cancer cells. circ-E2F3 promoted the tumor growth in vivo by inhibiting miR-296-5p.Conclusion circ-E2F3 played an important role in the progress of cervical cancer by inhibiting miR-296-5p. Highly-expressed circ-E2F3 may be an important indicator to predict the progress of cervical cancer.