CD226、CD96在非小细胞肺癌患者NK细胞中的表达及意义<sup>★</sup>

doi:10.3969/j.issn.2095-1264.2023.02.15

首页 > 过刊浏览>2023年第2期 >220-226. DOI:10.3969/j.issn.2095-1264.2023.02.15

CD226、CD96在非小细胞肺癌患者NK细胞中的表达及意义★
DOI:
                        10.3969/j.issn.2095-1264.2023.02.15
                    
作者:
                        
                        
                    
作者单位:河北燕达医院 呼吸内科，河北 三河，065201
作者简介:王立，男，副主任医师，研究方向：呼吸危重症及肺癌。
通讯作者:安阳，女，医师，研究方向：呼吸系统感染、呼吸内科。
中图分类号:R734.2
基金项目:★河北省2020年度医学科学研究课题（20200034）。

Expression and significance of CD226 and CD96 in NK cells of patients with non-small cell lung cancer^★

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Affiliation:

Department of Respiratory Medicine, Hebei Yanda Hospital, Sanhe, 065201, Hebei, China

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摘要:

目的探讨非小细胞肺癌（NSCLC）患者自然杀伤（NK）细胞中CD226、CD96的表达水平及意义。方法选择2016年1月—2017年1月我院收治的85例NSCLC患者为研究对象，选取同期在本院进行健康体检的50例正常人为对照。采用流式细胞术检测外周血NK细胞中活化受体CD226、CD96和NK细胞抑制性受体TIGIT的表达，并分析CD226、CD96与NSCLC患者临床病理特征的关系。采用Pearson分析CD226与CD96表达水平的关系；免疫组化法检测NSCLC组织和癌旁组织中CD226和CD96的共同配体CD155的表达，并分析CD155与NSCLC患者临床病理特征的关系。结果 NSCLC患者和正常人外周血NK细胞中CD226、CD96、TIGIT的阳性表达率分别为（75.32±12.02）% vs. （84.23±4.01）%（P<0.05），（25.31±5.36）% vs. （34.26±10.23）%（P<0.05），（47.36±20.08）% vs. （52.31±18.36）%（P>0.05）。Pearson分析结果显示，CD226⁺ NK细胞比例与CD96⁺ NK细胞比例呈正相关（r =0.486， P<0.05）。NSCLC高/中分化患者CD226⁺ NK细胞比例明显高于低分化患者（P<0.05）。无淋巴结转移患者CD96⁺ NK细胞比例明显高于有淋巴结转移患者（P<0.05）。NSCLC组织中CD155免疫组化染色总分明显高于癌旁组织（P<0.05），CD155的表达水平与患者临床病理特征无明显相关性（P>0.05）。结论 NSCLC患者NK细胞中CD226、CD96的表达水平均明显降低，这可能对肿瘤免疫逃逸有一定影响；上调NK细胞中CD226和CD96的表达可能成为治疗CD155阳性NSCLC患者的潜在方法。

Abstract:

Objective To investigate the expression and significance of CD226 and CD96 in natural killer (NK) cells of patients with non-small cell lung cancer (NSCLC).Methods Between January 2016 and January 2017, 85 patients with NSCLC in our hospital were selected as the research objects, and 50 normal persons who underwent physical examination in our hospital during the same period were selected as the control group. Flow cytometry was used to detect the expressions of activating receptors CD226, CD96 and inhibitory receptor TIGIT in peripheral blood NK cells. The relationship between CD226, CD96 and clinicopathological features of NSCLC was analyzed. Pearson method was used to analyze the relationship between CD226 and CD96 expression levels. The co-ligand CD155 of CD226 and CD96 in NSCLC and adjacent tissues were detected by immunohistochemistry. The relationship between CD155 and clinicopathological features of NSCLC was also analyzed.Results The positive rates of CD226, CD96 and TIGIT in peripheral blood NK cells of NSCLC patients and healthy persons were respectively (75.32±12.02)% vs. (84.23±4.01)% (P<0.05), (25.31±5.36)% vs. (34.26±10.23)% (P<0.05), (47.36±20.08)% vs. (52.31±18.36)% (P>0.05). Pearson analysis showed that the ratio of CD226⁺ NK cells was positively correlated with that of CD96⁺ NK cells (r=0.486, P<0.05). The percentage of CD226⁺ NK cells in NSCLC patients with high/moderate differentiation was significantly higher than that in patients with low differentiation (P<0.05). The percentage of CD96⁺ NK cells in patients without lymph node metastasis was significantly higher than that in patients with lymph node metastasis (P<0.05). The total score of CD155 immunohistochemical staining in NSCLC tissues was significantly higher than that in adjacent tissues (P<0.05). The expression of CD155 was not related to the clinicopathological features (P>0.05).Conclusion The expression levels of CD226 and CD96 in NK cells of NSCLC patients were significantly decreased, which may have some influence on the immune escape of tumors. Increasing the expressions of CD226 and CD96 in NK cells may be a potential therapy for NSCLC patients with CD155 positive expression.