TAT-tCNTF and CD affected cell viability by CCK-8. Using fluorescence microscope observed changes of F-actin resulted by CD and TAT-tCNTF. Level of intracellular free calcium concentration was detected by Fluo 4-AM loading through the fluorescence microscope and flow cytometry. Western blot detect F-actin protein expression.
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张琴,刘泽源,曲恒燕. TAT-tCNTF 对CD 诱导HUVEC 细胞损伤的作用研究(摘要)[J].肿瘤药学,2013,(6): ( in Chinese)