Objective Venenum Bufonis injection was a water-soluble extract of the traditional Chinese medicine Venenum Bufonis. It has great pharmacological effects like heat-clearing and detoxification, anti-inflammation, immunity-enhancing, antitumor and relieving cough and asthma. Some researches showed that the Venenum Bufonis combined with chemotherapy could improve the curative effect on advanced non-small cell lung cancer. This study made a meta-analysis on Venenum Bufonis combined with chemotherapy in the treatment of non-small cell lung cancer. Methods Database as CNKI, MEDLINE, EMBASE and so on were searched for“ Venenum Bufonis injection”,“ Toad venom”,“ Huachansu”,“ bufalin”,“ non small-cell lung cancer”,“ clinical trial”, etc. The final retrieval date: May 10, 2013. There were four articles in accordance with the requirements of article for meta-analysis. Results Venenum Bufonis injection could enhance the curative effect of non-small cell lung cancer chemotherapy, and improve the quality of life. But recent clinical studies existed obvious bias, so the results may be exaggerated.